TWO NEW SPECIES OF ALPINIA (ZINGIBERACEAE) FROM SULAWESI, INDONESIA

ARDI, W. H. & ARDIYANI, M. 2015. Two new species of Alpinia (Zingiberaceae) from Sulawesi, Indonesia. Reinwardtia14(2): 311 ‒ 316. — Two new species of Alpinia section Alpinia subsection Cenolophon, Alpinia macrocristaArdiyani & Ardi, and Alpinia pusilla Ardi & Ardiyani, from Sulawesi, Indonesia, are described. Colour plates are provided,and four-locus DNA barcodes have been generated for the purpose of identification. Tabulated key to species ofAlpinia subsection Cenolophon in Sulawesi is also presented

Keragaman Genetik Tacca Leontopetaloides (L.) Kuntze (Taccaceae) Dari Beberapa Provenansi Di Indonesia Berdasarkan Marka Inter Simple Sequence Repeats (Issr) [the Genetic Diversity of Tacca Leontopetaloides (L.) Kuntze (Taccaceae) From Various Provenance

Kecondang or Taka (Tacca leontopetaloides), a member of Taccaceae, is one the carbohydrate alternatives to rice which can be developed in coastal area. With the global warming, the sea level has increased and small islands are more isolated. The people who lives in these islands should depend on their bioresources.As Taka inhabits coastal area, the development of this plant becomes critical.A total of 65 accessions of Taka from 9 provenances in Indonesia (Sukabumi, Madura, Kp.Siung (Yogyakarta), Gn. Kidung (Yogyakarta), and the small islands in Karimun Jawa namely Nyamuk, Katang, Cendekia, Kumbang, Seruni islands) were characterized using six ISSR markers [GSGG (TG5)T; CCAG(TGG)3TG; GGAG(TGG)3TG; BDBT(TCC4); (AC)8C; (AG)8G]. The scoring of the fragments resulted in 55 (69.6%) polymorphic bands. The size of the bands varied from 150bp to 1,6kb. The 65 accessions of Taka made several clusters according to their provenances. The range of individual genetic dissimilarity was from 0.012 to 0.186. The results showed that amongst nine population studied, Gn. Kidul, Yogyakarta population has the highest level of genetic variation with mean values of Na = 1.3544±0.4814, Ne =1.1784±0.3025, PLP = 35.44% and He = 0.1094±0.1681. Whereas, P. Nyamuk, Kep. Karimun Jawa population has the lowest level of genetic variation with mean values of Na = 1.0759±0.2666, Ne = 1.0523±0.2163, PLP = 7.59% and He = 0.0275±0.1097

Typification and taxonomic notes of Smilacaceae species in Java

Smilacaceae is known as a taxa with wide phenotypic variation and their taxonomical complexities remain unsolved. The three species of Smilacaceae housed in Java, are given nomenclature history. Since all the potential sources of original material have been investigated, but nothing has been identified, three neotypifications were designated here. Herbarium specimen of Koorders 34990β in Herbarium Bogoriense (BO) was chosen as the neotype of Smilax klotzschii. The de Groot & Wehlburg RD52 herbarium specimen in BO was chosen as the neotype of S. nageliana and Blume 463 herbarium specimen in L was chosen as the neotype of S. odoratissima

ETLINGERA COMOSA, A NEW SPECIES (ZINGIBERACEAE: ALPINIOIDEAE) FROM CENTRAL SULAWESI

ARDIYANI, M., ARDI, W. H., HUTABARAT, P. W. K. & POULSEN, A. D. 2021. Etlingera comosa, a new species (Zingiberaceae: Alpinioidea) from Central Sulawesi. Reinwardtia 20(2): 63−68. — Etlingera comosa Ardiyani & Ardi, a new and unusual species from Tentena, Central Sulawesi exhibiting terrestrial as well as epiphytic habit is described here. It is compared to the morphologically closest Etlingera sublimata A.D.Poulsen, but differs in having tufted sheath, bilobed and asymmetric ligule, loose peduncular bracts, densely pubescent fertile bracts and longer filament. Colour plates, notes on its conservation status and DNA barcode data for the new species are also provided

KERAGAMAN GENETIK Tacca leontopetaloides (L.) Kuntze (Taccaceae) DARI BEBERAPA PROVENANSI DI INDONESIA BERDASARKAN MARKA INTER SIMPLE SEQUENCE REPEATS (ISSR)

Kecondang or Taka (Tacca leontopetaloides), a member of Taccaceae, is one the carbohydrate alternatives to rice which can be developed in coastal area. With the global warming, the sea level has increased and small islands are more isolated. The people who lives in these islands should depend on their bioresources.As Taka inhabits coastal area, the development of this plant becomes critical.A total of 65 accessions of Taka from 9 provenances in Indonesia (Sukabumi, Madura, Kp.Siung (Yogyakarta), Gn. Kidung (Yogyakarta), and the small islands in Karimun Jawa namely Nyamuk, Katang, Cendekia, Kumbang, Seruni islands) were characterized using six ISSR markers [GSGG (TG5)T; CCAG(TGG)3TG; GGAG(TGG)3TG; BDBT(TCC4); (AC)8C; (AG)8G]. The scoring of the fragments resulted in 55 (69.6%) polymorphic bands. The size of the bands varied from 150bp to 1,6kb. The 65 accessions of Taka made several clusters according to their provenances. The range of individual genetic dissimilarity was from 0.012 to 0.186. The results showed that amongst nine population studied, Gn. Kidul, Yogyakarta population has the highest level of genetic variation with mean values of Na = 1.3544±0.4814, Ne =1.1784±0.3025, PLP = 35.44% and He = 0.1094±0.1681. Whereas, P. Nyamuk, Kep. Karimun Jawa population has the lowest level of genetic variation with mean values of Na = 1.0759±0.2666, Ne = 1.0523±0.2163, PLP = 7.59% and He = 0.0275±0.1097

A NEW SPECIES OF ZINGIBER (ZINGIBERACEAE) FROM ENGGANO ISLAND, INDONESIA

ARDIYANI, M. 2015. - A new species of Zingiber (Zingiberaceae) from Enggano Island, Indonesia. Reinwardtia 14 (2): 307 - 310. - A species of Zingiber Miller (Zingiberaceae), Z. engganoensis Ardiyani, from Enggano Island, Indonesia is described. The species is only known from its type locality. It is similar to Z. spectabile Griff. but with some morphological differences. Three-locus DNA barcodes (rbcL, matK and ITS2) of the new species were generated for its identification purpose

Variasi Struktur Anatomi Daun Beberapa Jenis Alpinia Roxb. di Malesia

AbstrakKetidaksesuaian sistem klasifikasi Alpinia berdasarkan ciri morfologi dengan ciri molekuler menyebabkan sistem klasifikasi pada marga ini masih bermasalah. Data tambahan dari anatomi Alpinia diperlukan untuk mendukung sistem klasifikasi pada marga ini. Sayatan paradermal adaksial dan abaksial dari daun tujuh jenis Alpinia diamati. Sampel diambil dari koleksi Kebun Raya Bogor (KRB) yang mewakili jenis A. galanga, A. mutica, A. padacanca, A. macrocrista, A. malaccensis, A. pusilla, dan A. ligulata. Sebelas ciri Alpinia diamati seperti; ukuran stomata, jumlah stomata, indeks stomata, kerapatan stomata, tipe stomata, ukuran sel tetangga, jumlah sel tetangga, bentuk sel tetangga, ukuran epidermis, jumlah epidermis, dan bentuk epidermis. Hasil pengamatan menunjukkan bahwa beberapa ciri dapat digunakan untuk membedakan jenis Alpinia, seperti bentuk dan ukuran epidermis; ukuran, kerapatan dan indeks stomata; serta ukuran sel tetangga. Sementara ciri lainnya, seperti tipe stomata dan bentuk sel tetangga pada semua jenis Alpinia memiliki bentuk yang sama sehingga tidak dapat digunakan untuk membedakan jenis Alpinia.Abstract The incompatibility of Alpinia classification system based on morphological and molecular character causes classification system of this genus is still problematic. Additional data from Alpinia anatomy are needed to support the classification system in this genus. Adaxial and abaxial sides paradermal incisions of seven Alpinia species were observed. The samples were taken from the Bogor Botanical Gardens (KRB) collection representing A. galanga, A. mutica, A. padacanca, A. macrocrista, A. malaccensis, A. pusilla, and A. ligulata. Eleven Alpinia characteristics were observed; stomatal size, number of stomata, stomatal index, stomatal density, stomatal type, neighboring cells size, number of neighboring cells, neighboring cell shape, epidermal cells size, number of epidermal cells, and epidermal cells shape. The results showed that several characteristics can be used to distinguish Alpinia species, such as the shape and size of epidermal cells; the size, density and index of stomata; and the size of neighboring cells. Several other characteristics, such as the type of stomata and shape of neighboring cells in all species of Alpinia have the same shape so the characters cannot be used to distinguish Alpinia species.

TWO NEW SPECIES OF ALPINIA (ZINGIBERACEAE) FROM SULAWESI, INDONESIA

ARDI, W. H. & ARDIYANI, M. 2015. Two new species of Alpinia (Zingiberaceae) from Sulawesi, Indonesia. Reinwardtia 14(2): 311 - 316. - Two new species of Alpinia section Alpinia subsection Cenolophon, Alpinia macrocrista Ardiyani & Ardi, and Alpinia pusilla Ardi & Ardiyani, from Sulawesi, Indonesia, are described. Colour plates are provided, and four-locus DNA barcodes have been generated for the purpose of identification. Tabulated key to species of Alpinia subsection Cenolophon in Sulawesi is also presented